Functional analysis of putative genes encoding the PIP2 water channel subfamily in Populus trichocarpa.
Identifieur interne : 003624 ( Main/Exploration ); précédent : 003623; suivant : 003625Functional analysis of putative genes encoding the PIP2 water channel subfamily in Populus trichocarpa.
Auteurs : Francesca Secchi [États-Unis] ; Bryce Maciver ; Mark L. Zeidel ; Maciej A. ZwienieckiSource :
- Tree physiology [ 0829-318X ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Aquaporines (génétique), Aquaporines (métabolisme), Aquaporines (physiologie), Concentration en ions d'hydrogène (MeSH), Eau (métabolisme), Expression des gènes (effets des médicaments et des substances chimiques), Famille multigénique (physiologie), Métaux lourds (pharmacologie), Phylogenèse (MeSH), Populus (effets des médicaments et des substances chimiques), Populus (génétique), Populus (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Protéines végétales (physiologie), Transport biologique (génétique), Xenopus (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Expression des gènes, Populus.
- génétique : Aquaporines, Populus, Protéines végétales, Transport biologique.
- métabolisme : Aquaporines, Eau, Populus, Protéines végétales.
- pharmacologie : Métaux lourds.
- physiologie : Aquaporines, Famille multigénique, Protéines végétales.
- Animaux, Concentration en ions d'hydrogène, Phylogenèse, Xenopus.
English descriptors
- KwdEn :
- Animals (MeSH), Aquaporins (genetics), Aquaporins (metabolism), Aquaporins (physiology), Biological Transport (genetics), Gene Expression (drug effects), Hydrogen-Ion Concentration (MeSH), Metals, Heavy (pharmacology), Multigene Family (physiology), Phylogeny (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Proteins (physiology), Populus (drug effects), Populus (genetics), Populus (metabolism), Water (metabolism), Xenopus (MeSH).
- MESH :
- chemical , genetics : Aquaporins, Plant Proteins.
- chemical , metabolism : Aquaporins, Plant Proteins, Water.
- chemical , pharmacology : Metals, Heavy.
- chemical , physiology : Aquaporins, Plant Proteins.
- drug effects : Gene Expression, Populus.
- genetics : Biological Transport, Populus.
- metabolism : Populus.
- physiology : Multigene Family.
- Animals, Hydrogen-Ion Concentration, Phylogeny, Xenopus.
Abstract
We located fully sequenced putative genes of the plasma membrane intrinsic proteins (PIPs) family in the Populus trichocarpa (Torr. Gray), genome. Of 23 gene candidates, we assigned eight genes to the PIP2 subfamily. All eight putative genes were expressed in vegetative tissues (roots, leaves, bark and wood), and all of them showed water channel activity after being expressed in Xenopus oocytes. Six of eight proteins were affected by mercury ions. No proteins were affected by the presence of nickel or tungsten ions, or by lowering the pH of bathing external solution from 7.4 to 6.5. The presence of copper ions caused seven of eight PIP2 proteins to increase their water transport capacity by as much as 50%. This systematic study of the PIP2 subfamily of proteins in P. trichocarpa provides a basic overview of their activity as water channels and will be a useful reference for future physiological studies of plant water relations that use P. trichocarpa as a model system.
DOI: 10.1093/treephys/tpp060
PubMed: 19808706
Affiliations:
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Zeidel</name></author><author><name sortKey="Zwieniecki, Maciej A" sort="Zwieniecki, Maciej A" uniqKey="Zwieniecki M" first="Maciej A" last="Zwieniecki">Maciej A. 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Gray), genome. Of 23 gene candidates, we assigned eight genes to the PIP2 subfamily. All eight putative genes were expressed in vegetative tissues (roots, leaves, bark and wood), and all of them showed water channel activity after being expressed in Xenopus oocytes. Six of eight proteins were affected by mercury ions. No proteins were affected by the presence of nickel or tungsten ions, or by lowering the pH of bathing external solution from 7.4 to 6.5. The presence of copper ions caused seven of eight PIP2 proteins to increase their water transport capacity by as much as 50%. This systematic study of the PIP2 subfamily of proteins in P. trichocarpa provides a basic overview of their activity as water channels and will be a useful reference for future physiological studies of plant water relations that use P. trichocarpa as a model system.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19808706</PMID><DateCompleted><Year>2010</Year><Month>02</Month><Day>16</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0829-318X</ISSN><JournalIssue CitedMedium="Print"><Volume>29</Volume><Issue>11</Issue><PubDate><Year>2009</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Functional analysis of putative genes encoding the PIP2 water channel subfamily in Populus trichocarpa.</ArticleTitle><Pagination><MedlinePgn>1467-77</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpp060</ELocationID><Abstract><AbstractText>We located fully sequenced putative genes of the plasma membrane intrinsic proteins (PIPs) family in the Populus trichocarpa (Torr. Gray), genome. Of 23 gene candidates, we assigned eight genes to the PIP2 subfamily. All eight putative genes were expressed in vegetative tissues (roots, leaves, bark and wood), and all of them showed water channel activity after being expressed in Xenopus oocytes. Six of eight proteins were affected by mercury ions. No proteins were affected by the presence of nickel or tungsten ions, or by lowering the pH of bathing external solution from 7.4 to 6.5. The presence of copper ions caused seven of eight PIP2 proteins to increase their water transport capacity by as much as 50%. 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IdType="pii">tpp060</ArticleId><ArticleId IdType="doi">10.1093/treephys/tpp060</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Maciver, Bryce" sort="Maciver, Bryce" uniqKey="Maciver B" first="Bryce" last="Maciver">Bryce Maciver</name><name sortKey="Zeidel, Mark L" sort="Zeidel, Mark L" uniqKey="Zeidel M" first="Mark L" last="Zeidel">Mark L. Zeidel</name><name sortKey="Zwieniecki, Maciej A" sort="Zwieniecki, Maciej A" uniqKey="Zwieniecki M" first="Maciej A" last="Zwieniecki">Maciej A. Zwieniecki</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Secchi, Francesca" sort="Secchi, Francesca" uniqKey="Secchi F" first="Francesca" last="Secchi">Francesca Secchi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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